Immunofluorescent identification of human megakaryocyte colonies using an antiplatelet glycoprotein antiserum
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چکیده
منابع مشابه
Immunofluorescent identification of human megakaryocyte colonies using an antiplatelet glycoprotein antiserum.
The development of a satisfactory in vitro assay system for human megakaryocyte colony forming progenitor cells has been delayed by the lack of a suitable marker for cells of human megakaryocyte lineage. For this purpose we raised an antiserum directed against a purified human platelet glycoprotein preparation. In conjunction with indirect immunofluorescent staining of human bone marrow, this a...
متن کاملStudies of human megakaryocytopoiesis using an anti-megakaryocyte colony-stimulating factor antiserum.
We produced an antiserum by immunizing rabbits with purified human megakaryocyte colony stimulating factor (Meg-CSF). With the use of an anti-Meg-CSF IgG fraction (AM-IgG), we detected immunoreactive Meg-CSF both in human aplastic anemia serum (AAS) and normal serum. Based on our immunological and biological analyses, Meg-CSF appeared to be antigenically as well as functionally distinct from hu...
متن کاملInfluence of monoclonal antiplatelet glycoprotein antibodies on in vitro human megakaryocyte colony formation and proplatelet formation.
The influence of antiplatelet glycoprotein (GP) antibodies on megakaryocytopoiesis in patients with idiopathic or immune thrombocytopenic purpura (ITP) has been well studied. However, the influence of GP antibodies on proplatelet formation is poorly understood. Here we investigated whether in vitro human megakaryocyte colony formation and proplatelet formation are affected by various monoclonal...
متن کاملTwo monoclonal antiplatelet antibodies as markers of human megakaryocyte maturation: immunofluorescent staining and platelet peroxidase detection in megakaryocyte colonies and in in vivo cells from normal and leukemic patients.
Two monoclonal anti-human platelet antibodies have been used in an immunofluorescent assay to study megakaryoCyte maturation. The two antibodies were specific for platelets and megakaryocytes. AN 51 recognizes an antigen on platelet glycoprotein Ib; the antigen detected by J 1 5 is on the glycoprotein llb/Illa complex. since it does not bind to platelets from patients with Glanzmann’s thrombast...
متن کاملTwo Monoclonal Antiplatelet Antibodies as Markers of Human Megakaryocyte Maturation: Immunofluorescent Staining and Platelet Peroxidase Detection in Megakaryocyte Colonies and in In Vivo Cells From Normal and Leukemic Patients
Two monoclonal anti-human platelet antibodies have been used in an immunofluorescent assay to study megakaryoCyte maturation. The two antibodies were specific for platelets and megakaryocytes. AN 51 recognizes an antigen on platelet glycoprotein Ib; the antigen detected by J 1 5 is on the glycoprotein llb/Illa complex. since it does not bind to platelets from patients with Glanzmann’s thrombast...
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ژورنال
عنوان ژورنال: Blood
سال: 1981
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v57.2.277.277